Andrology-Open Access
Open Access

Chonticha Satirapod

Publications

• Research Article
  The Effects of Short Abstinence Time on Sperm Motility, Morphology and DNA mDamage
  Author(s): Matchuporn Sukprasert, Anna Wongkularb, Sasivimol Rattanasiri, Wicharn Choktanasiri and Chonticha SatirapodMatchuporn Sukprasert, Anna Wongkularb, Sasivimol Rattanasiri, Wicharn Choktanasiri and Chonticha Satirapod
  
  This was an experimental study to determine the influence of short abstinence time on sperm motility, morphology and DNA damage performed at the Assisted Reproductive Technology (ART) unit, Ramathibodi Hospital. Fifty-seven semen samples with normal semen analysis according to World Health Organization (WHO) 1999 and Kruger strict criteria were obtained. Volunteers were instructed to abstain for 3-5 days, 18-30 hours and 4 days for the 1st, 2nd and 3rd semen collection Total motile sperm count (TMSC) was determined using computer-assisted semen analysis (CASA); sperm morphology was determined using eosin-methylene blue staining and DNA damage was assessed by TUNEL assay. The results showed that TMSC was not significantly different between the 1st (3-5 days abstinence time) and 2nd semen collection (18-30 hours abstinence time after the 1st collection) (p v.. View More»
  DOI: 10.4172/2167-0250.1000107

  Abstract PDF

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