Gynecology & Obstetrics
Open Access

Choong-Chin Liew

Publications

• Research Article
  Use of Blood-based mRNA profiling to Identify Biomarkers for Ovarian Cancer Screening
  Author(s): Samuel C Mok, Jae-Hoon Kim, Steven J Skates, John O Schorge, Daniel W Cramer, Karen H Lu, Choong-Chin Liew Samuel C Mok, Jae-Hoon Kim, Steven J Skates, John O Schorge, Daniel W Cramer, Karen H Lu, Choong-Chin Liew
  
  Purpose: To identify candidate genomic signatures for the early detection of ovarian cancer using whole bloodbased gene expression profiles. Experimental Design: We performed Affymetrix U133Plus 2.0 GeneChip microarray analyses on whole blood RNA samples obtained from 14 ovarian cancer patients and 15 age-matched, healthy women. Genes differentially expressed were identified using a parametric Welch t-test. Real-time qRT-PCR analyses were performed on RNA prepared from 96 ovarian cancer patients and 83 age-matched healthy women, using primer sets specific for 14 genes. A Mann Whitney U test assessed individual gene significance. CA125 levels were determined in the same set of samples. We used logistic regression analyses and cross validation to assess the ability of linear combinations of specific transcripts combined with CA125 to distingui.. View More»
  DOI: 10.4172/2161-0932.1000443

  Abstract PDF

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