Clinical & Experimental Cardiology
Open Access

Chrissa P. Mower

Publications

• Research Article
  Common Variants in 6 Lipid-Related Genes Discovered by High-Resolution DNA Melting Analysis and Their Association with Plasma Lipids
  Author(s): John F. Carlquist, Jason T. McKinney, Benjamin D. Horne, Nicola J. Camp, Lisa Cannon-Albright, Joseph B. Muhlestein, Paul Hopkins, Jessica L. Clarke, Chrissa P. Mower, James J. Park, Zachary P. Nicholas, John A. Huntinghouse and Jeffrey L. AndersonJohn F. Carlquist, Jason T. McKinney, Benjamin D. Horne, Nicola J. Camp, Lisa Cannon-Albright, Joseph B. Muhlestein, Paul Hopkins, Jessica L. Clarke, Chrissa P. Mower, James J. Park, Zachary P. Nicholas, John A. Huntinghouse and Jeffrey L. Anderson
  
  Background: Total cholesterol was among the earliest identified risk factors for coronary heart disease (CHD). We sought to identify genetic variants in six genes associated with lipid metabolism and estimate their respective contribution to risk for CHD. Methods: For 6 lipid-associated genes ( LCAT, CETP , LIPC , LPL , SCARB1 , and ApoF ) we scanned exons, 5’ and 3’ untranslated regions, and donor and acceptor splice sites for variants using Hi-Res Melting® curve analysis (HRMCA) with confirmation by cycle sequencing. Healthy subjects were used for SNP discovery (n=64), haplotype determination/tagging SNP discovery (n=339), and lipid association testing (n=786). Results: In 17,840 bases of interrogated sequence, 90 variant SNPs were identified; 19 (21.1%) previously unreported. Thirty-four variants (3.. View More»
  DOI: 10.4172/2155-9880.1000138

  Abstract PDF

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