Journal of Hematology & Thromboembolic Diseases
Open Access
ISSN: 2329-8790
+44 1478 350008
ISSN: 2329-8790
+44 1478 350008
Claus L. Jensen
Denmark
Research Article
An Innovative Cis-Diammineplatinum (II) Benzene-Polycarboxylic-Acids Complex Inhibits Growth of Burkitt`s Cell Lymphoma In Vitro and In Vivo
Author(s): Steen Lindkær-Jensen, Claus L. Jensen, Naiel Azzam, Rinat Bar-Shalom, Basem Fares, Nina Habib L Jensen, Stig Larsen and Fuad FaresSteen Lindkær-Jensen, Claus L. Jensen, Naiel Azzam, Rinat Bar-Shalom, Basem Fares, Nina Habib L Jensen, Stig Larsen and Fuad Fares
Cis-diammineplatinum (II) benzene-polycarboxylic acids (CDBPA) is a new therapy of solid tumors without toxicity, mild and transient side-effects and an ability to improve quality of life. The underlying mechanism of action of CDBPA in Burkitt`s lymphoma was tested in vitro and its effect on tumor size and survival was tested in a NOD-scid mice model. BL cells and normal fibroblasts were exposed to CDBPA for cytotoxicity screening by XTT assay, followed by cell cycle arrest, determination of apoptosis using Flow Cytometry and by annexin V/FITC/PI assay. Protein level and Gene expression were tested by Western blotting analysis and by the Applied Biosystems� Tagman� Array Plates, respectively. We demonstrated that CDBPA significantly reduced cell viability and induced apoptosis via the extrinsic and the intrinsic apoptotic pathways of Burkitts lymphoma cells without causing cell cycle arr.. View More»
DOI:
10.4172/2329-8790.1000234