Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
+44 1478 350008
ISSN: 2155-9554
+44 1478 350008
Cuong Hoang-Vu
Department of General, Visceral and Vascular Surgery,
Martin-Luther-University Halle-Wittenberg
Germany
Research Article
Up-Regulation of 14-3-3σ in Hacat upon Co-Cultivation with HEPM
Author(s): Gabriel Magnucki, Joanna Białek, Kathrin Hammje, Hanna Prenzel, Gunter Klohs, Johannes Wohlrab, Cuong Hoang-Vu and Rainer FinkeGabriel Magnucki, Joanna Białek, Kathrin Hammje, Hanna Prenzel, Gunter Klohs, Johannes Wohlrab, Cuong Hoang-Vu and Rainer Finke
Background: To generate an in vitro system of the dermal epithelial-mesenchymal interaction we co‑cultured HaCaT (keratinocytes) and HEPM (embryonic mesenchyme). This model allowed a local disjunction with preserved cell-cell communication.
Methods: For the analysis of different protein expression patterns between co- and pure-cultured HaCaTs we performed 2D-electrophoresis and mass spectrometry. Afterwards the mass spectromertically identified protein 14-3- 3σ was silenced by siRNA in HaCaT cells.
Results: We analyzed 28 spots and found 17 different expressed mainly metabolic and cytoskeletal proteins. Interestingly, stratifin (14-3-3σ), maspin and Profilin-1 (PFN1) were up-regulated, whereas Peroxiredoxin-5 (PRDX5), PDZ-and-LIM-domain-protein-1 (CLIM1) and Annexin A1 (AN.. View More»
DOI:
10.4172/2155-9554.1000171