Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
+32 25889658
ISSN: 2155-9600
+32 25889658
Cynthia M Barber
Russia
Research Article
Immunoassay Platform for Casein Antigens in Hydrolyzed Casein-Based Infant Formula
Author(s): G. Christopher Butler, Michael B Miklus, Cynthia M Barber, Steven M Tennyson and Pedro A PrietoG. Christopher Butler, Michael B Miklus, Cynthia M Barber, Steven M Tennyson and Pedro A Prieto
Hypoallergenic formulas are the sole source of nutrition for infants that are either allergic to milk proteins or at risk of developing allergies. Strategies to provide nutritional sustenance, while preventing allergic reactions, include designing formulas based on extensively hydrolyzed casein which is presumably devoid of antigenic epitopes. Assays devoted to the assessment of antigenic protein motifs are crucial to verify the absence of relevant antigens in formulas and the raw materials used in their preparation. Evaluation of commercial immunoassay kits intended for the detection of milk proteins in foods led to the conclusion that a specific assay for extensively hydrolyzed casein-based formulas was necessary to improve allergen recoveries and assay consistency. The purpose of this investigation was to develop a reproducible path, from the generation of antibodies to the pre-val.. View More»