Journal of Plant Biochemistry & Physiology
Open Access

Dagnachew Bekele

Publications

• Review Article
  Applications of Virus Induced Gene Silencing (VIGS) in Plant Functional Genomics Studies
  Author(s): Dagnachew Bekele, Kassahun Tesfaye and Asnake FikreDagnachew Bekele, Kassahun Tesfaye and Asnake Fikre
  
  Virus induced gene silencing (VIGS) is an effective technology that exploits an antiviral defense mechanism in plants. It is a recently developed gene transcript suppression technique for characterizing the function of plant genes. VIGS is rapid, efficient and specific system for transient gene silencing. The major steps in VIGS includes; engineering viral genomes to the appropriate viral vector to incorporate fragments of host genes that are targeted to be silenced, infecting the appropriate plant hosts and silencing the target genes as part of the defense mechanism of the plant against virus infection. The VIGS vector is a recombinant virus engineered to be able to carry a piece an endogenous gene from the host. During infection with the modified vector, the host’s defense reaction will be induced against the cloned host gene; a loss of function phenotype makes it possible to .. View More»
  DOI: 10.4172/2329-9029.1000229

  Abstract PDF

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