Gynecology & Obstetrics
Open Access
ISSN: 2161-0932
ISSN: 2161-0932
Daniel W Cramer
Tanzania
Research Article
Use of Blood-based mRNA profiling to Identify Biomarkers for Ovarian Cancer Screening
Author(s): Samuel C Mok, Jae-Hoon Kim, Steven J Skates, John O Schorge, Daniel W Cramer, Karen H Lu, Choong-Chin Liew
Samuel C Mok, Jae-Hoon Kim, Steven J Skates, John O Schorge, Daniel W Cramer, Karen H Lu, Choong-Chin Liew
Purpose: To identify candidate genomic signatures for the early detection of ovarian cancer using whole bloodbased gene expression profiles.
Experimental Design: We performed Affymetrix U133Plus 2.0 GeneChip microarray analyses on whole blood RNA samples obtained from 14 ovarian cancer patients and 15 age-matched, healthy women. Genes differentially expressed were identified using a parametric Welch t-test. Real-time qRT-PCR analyses were performed on RNA prepared from 96 ovarian cancer patients and 83 age-matched healthy women, using primer sets specific for 14 genes. A Mann Whitney U test assessed individual gene significance. CA125 levels were determined in the same set of samples. We used logistic regression analyses and cross validation to assess the ability of linear combinations of specific transcripts combined with CA125 to distingui.. View More»
DOI:
10.4172/2161-0932.1000443