Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Danyel Smith
Tanzania
Research Article
Proteome Biomarkers in Xylem Reveal Pierce′s Disease Tolerance in
Grape
Author(s): Ramesh Katam, Kundai Chibanguza, Lekan M Latinwo and Danyel SmithRamesh Katam, Kundai Chibanguza, Lekan M Latinwo and Danyel Smith
Pierce’s disease (PD) is a significant threat to grape cultivation and industry. The disease caused by bacterium Xylella fastidiosa clogs xylem vessels resulting in wilting of the plant. PD-tolerant grape genotypes are believed to produce certain novel components in xylem tissue that help them to combat invading pathogens. Research has been aimed at characterizing the uniquely expressed xylem proteins by PD-tolerant genotypes. The objectives were to i) compare and characterize Vitis xylem proteins differentially expressed in PD-tolerant and PD-susceptible cultivars and, ii) identify xylem proteins uniquely expressed in PD-tolerant genotypes. A high throughput two-dimensional gel electrophoresis of xylem proteins from three Vitis species identified more than 200 proteins with pIs 3.0 to 9.0 and molecular weights of 20 to 75 kDa. The differentially expressed proteins were then exc.. View More»
DOI:
10.4172/jpb.1000372