Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
David M Lubman
1150 West Medical Center Drive, Building MSRB1 Room A510B, Ann Arbor, MI
Tanzania
Research Article
Heterogeneity of The CD90+ Population in Different Stages of
Hepatocarcinogenesis
Author(s): Smathorn Thakolwiboon, Jianhui Zhu, Qixing Liang, Theodore H Welling, Min Zhang and David M LubmanSmathorn Thakolwiboon, Jianhui Zhu, Qixing Liang, Theodore H Welling, Min Zhang and David M Lubman
We have characterized herein the heterogeneity of the CD90+ population at each stage of hepatocarcinogenesis using a computer-assisted immunohistochemical staining evaluation method for quantitative analysis on tissue microarrays. We found that CD90 in Hepatocellular carcinoma (HCC) tissues, which has been shown to be a marker for cancer stem cells, is expressed on tumor cells, in the stroma or on endothelial cells. Sub-classification of the CD90+ population was based on morphology and co-expression with known markers including CD45 and CD31. Multiple linear regression suggested that the percentage of CD90+ cancer cells/hepatocyte (p<0.0001), level of overall CD90 expression (p<0.0014), and level of CD90 expression in tumor islands (p<0.0001) increased significantly in each stage of liver disease progression, while the level of stromal CD90 expression (p=0.1129) did not chang.. View More»
DOI:
10.4172/jpb.1000332