Advanced Techniques in Biology & Medicine
Open Access
ISSN: 2379-1764
ISSN: 2379-1764
Debabrat Sabat
India
Special Issue Article
A Protocol to Generate Germ Free Drosophila for Microbial Interaction
Studies
Author(s): Debabrat Sabat, Eldin M Johnson, Arra Abhinay, Rasu Jayabalan and Monalisa MishraDebabrat Sabat, Eldin M Johnson, Arra Abhinay, Rasu Jayabalan and Monalisa Mishra
Drosophila (fly) gut is used as a model system to study the host microbe interaction. Various developmental, physiological, functional and immunological similarities exist between the gut of the vertebrate and the fly. These similarities advocates their utility as a model system for the study of various gut related diseases. Wild type flies found in its natural environment and those raised in the enclosed laboratory conditions have their own natural microbiota colonizing the gut. This natural gut microbiota may interfere with orally administered microbe-Drosophila interaction studies. To overcome this problem various labs are in the process of raising germ free flies. Germ free Drosophila is completely devoid of intestinal microbiota or any kind of microbial colonization. These germ free fly would be helpful as powerful model in exploring the role of a particular microbial strain or a.. View More»
DOI:
10.4172/2379-1764.S1-001