Cell & Developmental Biology
Open Access

Denika D Rhodes

Publications

• Research Article
  Osmotic Stress Modulates the Expression and Function of the Human LDL Receptor
  Author(s): Catherine J Wooten, Ayodele Fakayode, Audrey F Adcock, Kendra M Davis, Raven S King, Denika D Rhodes and Dayami Lopez Catherine J Wooten, Ayodele Fakayode, Audrey F Adcock, Kendra M Davis, Raven S King, Denika D Rhodes and Dayami Lopez
  
  The purpose of this study was to determine whether the Low-Density Lipoprotein (LDL) receptor could be directly regulated by high glucose (HG) in human hepatocyte-like C3A cells. C3A cells were cultured in a medium supplemented with BD™ MITO+ serum extender (MITO+ medium), a serum-free, cholesterol deficient medium. We found that HG reduced receptor mRNA levels without significantly affecting overall or plasma membrane receptor protein expression. Interestingly, these effects were also seen in the presence of low glucose + high mannitol (LG +HM). LDL receptor protein synthesis, protein degradation, and receptor function (LDL internalization) were increased by HG and LG+HM. However, no changes in protein expression of proprotein convertase subtilisin kexin type 9 (PCSK9) or the inducible degrader of LDL receptors (IDOL), the known degraders of the LDL receptor, were seen under th.. View More»
  DOI: 10.4172/2168-9296.1000147

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