Journal of Plant Biochemistry & Physiology
Open Access

Denise Medrano

Publications

• Research Article
  Medicago sativa L. β-Amylase Core Promoter has Motifs in Common with Arabidopsis Key Starch Degradation Genes
  Author(s): Brett Fugate, Denise Medrano, Joyce Ache Gana, Aida Abraha and Suzanne M Cunningham Brett Fugate, Denise Medrano, Joyce Ache Gana, Aida Abraha and Suzanne M Cunningham
  
  β-amylase is an enzyme involved in the degradation of transitory starch in photosynthetic tissues of plants and is expressed in high levels in the roots of Medicago sativa L. How the β -amylase gene MsBAM1 is regulated in the roots is not clear. The aims of this study were to: isolate the core promoter of MsBAM1, decipher motifs in silico that are associated with the elevated root specific response, and to find common motifs among core promoters of important starch degradation genes in Medicago sativa MsBAM1 and Arabidopsis (AtGWD, AtPWD, AtMEX1, AtBAM 1 & 3, and AtISA3). Primers were designed from homologous β-amylase genes and used in PCR with M. sativa gDNA. Gel purified PCR band(s) were sequenced, verified and annotated. The starch genes and equal numbers of Arabidopsis non-starch.. View More»
  DOI: 10.4172/2329-9029.1000124

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