Journal of Glycobiology
Open Access

Douglas A Weidner

Publications

• Research Article
  Membrane Distribution and Activity of a Neuronal Voltage-Gated K+ Channel is Modified by Replacement of Complex Type N-Glycans with Hybrid Type
  Author(s): M Kristen Hall1, Douglas A Weidner2, Sahil Dayal1, Elena Pak3, Alexander K Murashov3 and Ruth A Schwalbe1*M Kristen Hall1, Douglas A Weidner2, Sahil Dayal1, Elena Pak3, Alexander K Murashov3 and Ruth A Schwalbe1*
  
  Abnormal modifications in N-glycosylation processing are commonly associated with neurological disorders, although the impact of specific N-glycans on neuronal excitability is unknown. By replacement of complex types of N-glycans with hybrid types in neuroblastoma cells, we provide the first study that addresses how distinct N-glycan types impact neuronal excitability. Using CRISPR/Cas9 technology, NB_1, a clonal cell line derived from rat neuroblastoma cells (NB), was modified to create an N-glycosylation mutant cell line, NB_1 (-Mgat2), which expresses predominantly hybrid type N-glycans. Western and lectin blotting, flow cytometry, TIRF and DIC microscopy, and patch clamp studies were conducted. Lectin binding revealed the predominant type of N-glycans expressed in NB_1 (-Mgat2) is hybrid while those of NB and NB_1 are complex. Kv3.1b-expressing cells with complex N-glycans localiz.. View More»
  DOI: 10.4172/2168-958X.1000128

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