Journal of Down Syndrome & Chromosome Abnormalities
Open Access

Eleanor Feingold

Publications

• Research Article
  Variation in the Zinc Finger of PRDM9 is Associated with the Absence of Recombination along Nondisjoined Chromosomes 21 of Maternal Origin
  Author(s): Tiffany Renee Oliver, Candace Middlebrooks, Ariel Harden, Nyeisha Scott, Blair Johnson, Jillian Jones, Christin Walker, Corinthia Wilkerson, Sha- Hanna Saffold, Abisola Akinseye, Tunde Smith, Eleanor Feingold and Stephanie L ShermanTiffany Renee Oliver, Candace Middlebrooks, Ariel Harden, Nyeisha Scott, Blair Johnson, Jillian Jones, Christin Walker, Corinthia Wilkerson, Sha- Hanna Saffold, Abisola Akinseye, Tunde Smith, Eleanor Feingold and Stephanie L Sherman
  
  Variation in the zinc finger-binding domain (ZFBD) of the protein PR Domain-Containing Protein 9 (PRDM9) is associated with altered placement of recombination in the human genome. As both the absence and altered placement of recombination are observed among chromosomes 21 that nondisjoin, we genotyped the PRDM9 ZFBD among mothers of children with Trisomy 21 in efforts to determine if variation within this region is associated with the recombination-related risk for chromosome 21 nondisjunction (NDJ). In our approach, PCR was used to amplify the ZFBD of PRDM9 and products were then subjected to bi-directional Sanger sequencing. DNA sequencing reads were aligned and compared to the sequence of the PRDM9 alleles previously identified. Chi-Square analysis was used to compare allele frequencies between cases (N=235, mothers of children with maternally-derived Trisomy 21) and controls (N=48.. View More»
  DOI: 10.4172/2472-1115.1000115

  Abstract PDF

Relevant Topics