Advances in dairy Research
Open Access

Elisha D Johnson

Publications

• Research Article
  Differences in Total Protein Concentration between Fresh and Frozen Serum and Plasma Samples Used to Assess Failure of Passive Transfer in Dairy Calves
  Author(s): Aurora Villarroel, Taylor B Miller, Justin K Ward, Elisha D Johnson and Kimberly R Noyes Aurora Villarroel, Taylor B Miller, Justin K Ward, Elisha D Johnson and Kimberly R Noyes
  
  The objectives of this study were to determine differences in total protein (TP) concentration between samples before and after freezing, and to determine the correlation between total protein in serum and plasma to evaluate passive transfer in calves. Blood was collected via jugular vein puncture from a total of 127 calves (34 Holstein and 93 Jersey) once between 1 and 3 days of age. Serum and plasma protein concentrations were measured via digital refractometer in fresh specimens; samples were then frozen for storage. After a variable period of time in storage (≤1 to 8 months), frozen samples were thawed and re-analyzed. Passing-Bablok regression showed that total protein concentration in plasma was consistently higher than in serum. Additionally, multivariate regression showed that Jersey calves had higher total protein concentration in serum than Holstein calves (0.429 ±.. View More»
  DOI: 10.4172/2329-888X.1000111

  Abstract PDF

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