Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Ellen Meijer
Netherlands
Research Article
Cytomegalovirus (CMV)-Reactivation Influences T-Cell Differentiation and CMV-Specific T-Cell Reconstitution after Stem Cell Transplantation
Author(s): Jolanda Scherrenburg, Floor Pietersma, Ronald Jacobi, Rob Schuurman, Ellen Meijer and Debbie van BaarleJolanda Scherrenburg, Floor Pietersma, Ronald Jacobi, Rob Schuurman, Ellen Meijer and Debbie van Baarle
CMV-specific T cells were shown to be important for protection against CMV-disease in SCT recipients. Here we investigated specific T-cell features like effector cell differentiation and perforin-expression as well as CMV-specific T cells after SCT in relation to CMV-reactivation. To this end, CD4+ and CD8+ T-cell characteristics (differentiation, activation and functional CMV-specific immunity) of SCT patients with (n=13) or without (n=8) CMV-reactivation were analysed longitudinally by flow cytometry. CMV-specific IFNγ-production as measured by intracellular staining and proliferation as measured by CFSE dye dilution were analysed after stimulation with overlapping peptide pools of the tegument protein pp65 and immediate early antigen 1. A more differentiated phenotype, up-regulation of the activation markers CD38 and HLA-DR on CD4+ T cells and increased expression of perforin .. View More»
DOI:
10.4172/2155-9899.S9-001