Journal of Pollution Effects & Control
Open Access
ISSN: 2375-4397
ISSN: 2375-4397
Eman Saleh Farrag
Slovak republic
Research Article
Detection of Extended Spectrum Beta-Lactamase Producing Escherichia Coli on Water at Hafr Al Batin, Saudi Arabia
Author(s): Sulaiman Ali Al Yousef, Eman Saleh Farrag, Ahmed Mohamed Ali and Sabry Younis Mahmoud
Sulaiman Ali Al Yousef, Eman Saleh Farrag, Ahmed Mohamed Ali and Sabry Younis Mahmoud
In this study antibiotic resistant Escherichia coli from household water collected in Hafr AlBatin, Saudi Arabia, were characterized by prevalence of extended spectrum betalactamase (ESBL). Samples were collected from drinking and washing water at 12 locations. From the 144 samples obtained, Millipore membrane filters incubated on trypton soya agar plates produced colonies that yielded 34 isolates of E. coli as verified by biochemical tests. Isolates suspected ESBL producing were tested by using MicroScan analysis and disc diffusion test as ESBL phenotypic confirmatory methods. Phenotypically confirmed ESBL isolates were examined for antimicrobial susceptibility against 30 antibiotics and amplification of blaVEM, blaCTX, blaTEM, blaGES and blaSHV genes by polymerase chain reaction. Out of 34 E. coli isolates, only 6 (17.6%) were positive for ESBL producing according t.. View More»
DOI:
10.4172/2375-4397.1000155