Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Eva M Abad-Villar
Switzerland
Research Article
Corneal Penetration of Polyhexamethylene Biguanide and Chlorhexidine Digluconate
Author(s): Susanne F Vontobel, Eva M Abad-Villar, Claude Kaufmann, Annelies S Zinkernagel, Peter C Hauser and Michael A ThielSusanne F Vontobel, Eva M Abad-Villar, Claude Kaufmann, Annelies S Zinkernagel, Peter C Hauser and Michael A Thiel
Objective: Cationic antiseptics, such as polyhexamethylene biguanide (PHMB) and chlorhexidine digluconate (CHG), are widely used for the topical treatment of Acanthamoeba keratitis. The aim of this study was to investigate the corneal penetration of PHMB and CHG topically administered as eye drops and to study the effect of PHMB and CHG on the epithelial barrier function. Methods: The penetration was evaluated in vitro in rabbit corneas clamped in artificial perfusion chambers. Two different preparations of PHMB 0.02% (Cosmocil and Lavasept) and CHG 0.02% eye drops were administered twice each hour for up to 8 hours to the rabbit corneas with and without epithelium. The amount of drug penetrating into the anterior chamber was measured using capillary electrophoresis with contactless conductivity detection. The integrity of the epithelial barrie.. View More»
DOI:
10.4172/2155-9570.1000430