Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Ewa Sitkiewicz
Poland
Research Article
Differential Quantitative Proteomics of Human Microvascular Endothelial Cells 1 by iTRAQ Reveals Palladin to be a New Biomarker During TGF-?1 Induced Endothelial Mesenchymal Transition
Author(s): Marta Stasiak, Katarzyna Gawrys, Marcin Popielarski, Radoslaw Bednarek, Maciej Studzian, Ewa Sitkiewicz, Janusz Szemraj and Maria Swiatkowska
Marta Stasiak, Katarzyna Gawrys, Marcin Popielarski, Radoslaw Bednarek, Maciej Studzian, Ewa Sitkiewicz, Janusz Szemraj and Maria Swiatkowska
The study uses global quantitative proteomics to investigate the molecular mechanisms behind the induction of endothelial-mesenchymal transition (EndMT) by transforming growth factor–β (TGF-β). Orbitrap Velos mass spectrometers and iTRAQ – a labeling-based analysis were used to perform a global and quantitative comparison of two proteomes of Human Microvascular Endothelial Cells-1 (HMEC-1) treated or not treated by TGF-β1. iTRAQ analysis identified 43 differentially-expressed proteins in the early stages of EndMT induced by TGF-β1. From 5522 identified proteins, 26 were downregulated and 17 were upregulated, including proteins such as palladin, POTE I, torsin A and nucleoporin (NDC1). Further analysis of palladin revealed its increased mRNA and protein expression in response to TGF-β and Snail transcription factor. Our findings demonstrate that the.. View More»
DOI:
10.4172/jpb.1000447