Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Eyal Grunebaum
Hospital for Sick Children,
555 University Avenue, Toronto, Ontario, M5G 1X8
Canada
Research Article
Regulatory T cells Suppress Zap70 Phosphorylation in Responding T cells
Author(s): Yoram Faitelson, Weixian Min and Eyal Grunebaum
Yoram Faitelson, Weixian Min and Eyal Grunebaum
Objective: Regulatory T cells (Treg) have a critical role in controlling responding T cells (Tresp), thereby preventing autoimmune manifestations including those observed in adenosine deaminase (ADA) enzyme-deficient patients and mice (ADA-KO). Treg suppress various functions of Tresp such as proliferation, cytokine production and expression of activation markers, yet it is not known if Treg suppress the early steps of Tresp activation, such as the phosphorylation of the Zeta-associated protein (Zap)70.
Methods: Zap70 phosphorylation and CD69 expression in anti-CD3 and anti-CD28 stimulated mice CD4+CD25- Tresp were measured by flow cytometry in the presence or absence of CD4+CD25+ Treg activated with anti-CD3 and anti-CD28 antibodies. Suppression of Zap70 phosphorylation in Tresp from normal mice cult.. View More»
DOI:
10.4172/2155-9899.1000440