Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Eyasu Makonnen
Ethiopia
Research Article
In-vitro Investigation of Fractionated Extracts of Albizia gummifera Seed Against Leishmania donovani Amastigote Stage
Author(s): Dereje Nigussie, Geremew Tasew, Eyasu Makonnen, Asfaw Debella, Birhanu Hurrisa, Kelbessa Urga and Adugna WayessaDereje Nigussie, Geremew Tasew, Eyasu Makonnen, Asfaw Debella, Birhanu Hurrisa, Kelbessa Urga and Adugna Wayessa
Natural products represent a rich and promising source of novel, biologically active chemical entities for treating Leishmaniasis. This study investigates the anti-leishmanial activity of n-butanol, aqueous, and chloroform fractions obtained from crude methanolic extract of Albizia gummifera seed in in-vitro (peritoneal mice macrophage) model after one time treatment. The anti-lesihmanial activity was determined from the minimum inhibitory concentration (MIC), infection rate (IR) and multiplication index (MI) against L. donovani. Cyto-toxicity of the fractions was also assessed against Vero cells. In addition to this secondary metabolites were determined by phytochemical screening. The MIC value of n-butanol, aqueous, chloroform and amphotericin B were 11.2 μg/ml, 33.5 μg/ml, >89 μg/ml and 1.32 μg/ml, respectively. N-butanol a.. View More»
DOI:
10.4172/2155-9899.1000373