ISSN: 2593-9173
Ezzat Abuzaed
Egypt
Research Article
Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita
Author(s): Khaled Elbanna, Hosny Gamal-Eldin and Ezzat Abuzaed
Khaled Elbanna, Hosny Gamal-Eldin and Ezzat Abuzaed
A total of 52 Egyptian rhizosphere fluorescent pseudomonad isolates (ERFP) were screened for their nematicidal activity in vitro. The screening results showed an inhibitory effect on hatching of Meloidogyne incognita eggs ranging from 57 % to 100 %. Similar to the chemical nematicide Videt in vitro experiment, cultures or cell-free supernatants (CFS) of the ERFP isolates showed complete inhibition of egg hatching and killed 100 % of the J2. In glasshouse pot experiments, cultures of these isolates, CFS and the chemical nematicide showed reduction in root galling ranging from 81.0-95.4, 61.3-84.5, 97.3%, respectively. Also, the reduction in nematode multiplication in soil was 91.9-95.7, 83.5-84.5 and 96.5% for cultures, CFS and the chemical nematicide, respectively, compared to the positive control (nematode only). Furthermore, these isolates showed significant increases in plant growt.. View More»
DOI:
10.4172/2155-6202.1000102