Anatomy & Physiology: Current Research
Open Access
ISSN: 2161-0940
+44 1300 500008
ISSN: 2161-0940
+44 1300 500008
Research Article
Acute Experimental Diabetes and Aortic Depressor Nerve Ultrastructural Morphometry: Effects of Insulin Treatment
Author(s): Fabrício Singaretti de Oliveira, Milena Menezes de Amorim, Jaci Airton Castania, Helio Cesar Salgado, Randy Alan Nessler, Valéria Paula Sassoli Fazan
Fabrício Singaretti de Oliveira, Milena Menezes de Amorim, Jaci Airton Castania, Helio Cesar Salgado, Randy Alan Nessler, Valéria Paula Sassoli Fazan
Previous results from our laboratory showed myelinated fiber alterations in the aortic depressor nerve (ADN) from rats rendered diabetic for 15 days (short term). The ultrastructure and the acute effect of insulin treatment, particularly in the unmyelinated fibres of this nerve, were not yet investigated in this experimental model, being the aim of this work. Wistar rats received a single intravenous injection of streptozotocin (STZ – 40 mg/Kg) 15 days (N=7) before the experiments. Control animals (N=7) received vehicle (citrate buffer) and treated animals (n=6) received a subcutaneous injection of insulin (10 IU/Kg) on a daily basis beginning 3 days after the STZ injection. Under pentobarbital anaesthesia the ADNs were isolated and had their spontaneous activity recorded. Afterwards, proximal and distal segments of the nerves were prepared for transmission electron microscopy s.. View More»
DOI:
10.4172/2161-0940.1000159