Journal of Chromatography & Separation Techniques
Open Access

Falgun A Mehta

Publications

• Research Article
  Simultaneous Estimation of Ambroxol Hydrochloride and Doxofylline in Pharmaceutical Formulation by HPTLC-Desitometric Method
  Author(s): Falgun A Mehta, Emanual Michael Patelia and Pradip N Bhoya Falgun A Mehta, Emanual Michael Patelia and Pradip N Bhoya
  
  A simple, precise, rapid, selective, and economic reversed phase high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of Ambroxol Hydrochloride and Doxofylline. The HPTLC method was performed on precoated silica gel G60 F254 plates with Diethyl ether:n-butanol:Ammonia (9:0.9:0.1 v/v/v) as mobile phase. The plates were developed in a 7.0 cm at ambient temperature. The developed plates were scanned and quantified at their absorption at approximately 254 nm for AMB and DOX. The drugs were satisfactorily resolved with Rf 0.29 ± 0.02 for and Rf 0.56 ± 0.02 for DOX. The calibration plot was linear between 100- 600 ng /band for AMB and 200-1200 ng/band based for DOX. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis.. View More»
  DOI: 10.4172/2157-7064.1000168

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