Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Francisco Obregón
Venezuela
Research Article
Extracellular Zinc Chelator In Vivo On System Of Taurine in Retina: Transport, Concentrations and Localization of Transporter
Author(s): Asarí Márquez, Mary Urbina, Manuelita Quintal, Francisco Obregón, Víctor Salazar, Lucimey Lim
Asarí Márquez, Mary Urbina, Manuelita Quintal, Francisco Obregón, Víctor Salazar, Lucimey Lim
Taurine and zinc are relevant molecules in the retina, both are highly concentrated and have similar functions in this structure. Little is known about the mechanisms of action of this amino acid and the interaction with other molecules such as zinc. The objectives of the present study were to evaluate the consequences of zinc deficiency by the extracellular chelator diethylenetriaminepenta-acetic acid (DTPA) on taurine levels, transport and localization of taurine transporter in rat retina. Various concentrations of the extracellular zinc chelator, DTPA, dissolved in dimethylsulfoxide, was administered intraocular: 10, 100, 250 and 500 μM. Retinas were dissected 3, 5 and 10 days later. Zinc was determined by spectrophotometry. Taurine levels, determined by high performance liquid chromatography with fluorescence detection, DTPA administration, 10 μM, decreased taurine in 56% at.. View More»
DOI:
10.4172/2155-9570.1000662