Journal of Nutrition & Food Sciences
Open Access

Freddy Havaldar

Publications

• Research Article
  An In Vitro Study on the Effect of Five Commercial Calcium Supplements on Human Osteoblast Cell Proliferation and Ca²⁺ Mineralization
  Author(s): Bomi Framroze and Freddy Havaldar Bomi Framroze and Freddy Havaldar
  
  Calcium is an essential mineral that supports bone and joint health. It is used as a supplement, most typically as calcium carbonate, to assist in preserving bone density especially when dietary calcium intake is inadequate. In the present study we examined the effect of five varied calcium sources, three natural and two synthetic, on stimulation, proliferation and mineralization of cultured human osteoblast cells. A MTT assay using cultured human fetal osteoblast cells (hFOB 1.19) was used to determine the effective proliferative dose for salmon collagen bone calcium (0.25 mg/ml). Elemental calcium equivalence was used to select the appropriate doses for algae calcium (0.14 mg/ml), eggshell powder (0.16 mg/ml), calcium citrate (0.22 mg/ml) and calcium carbonate (0.13). Alkaline phosphatase activity, DNA synthesis rates and calcium ion deposition were evaluated after incubation under .. View More»
  DOI: 10.4172/2155-9600.1000738

  Abstract PDF

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