ISSN: 0974-276X
Fu-Rong Li
The Key Laboratory of Stem Cell and Cellular Therapy, The Second Clinical Medical College (Shenzhen People’s Hospital),
No.1017 Dongmen North Road, Shenzhen 518020
China
Research Article
Discovery of Novel Proteins form Injured Rat Pancreatic Extract using MALDI-TOF/MS-based Proteomics
Author(s): Hongbin Xie, Hui Zhang, Hui Qi, Yunshuai Wang, Chun-Yan Deng and Fu-Rong Li
Hongbin Xie, Hui Zhang, Hui Qi, Yunshuai Wang, Chun-Yan Deng and Fu-Rong Li
Injured pancreatic tissue extract contains transcription proteins that are considered as specific soluble proteins, which contribute in promoting the trans-differentiation of stem cells into insulin-producing cells (IPCs). In this present study, 60% of the pancreatic tissues of Sprague-Dawley (SD) rats were removed, and newborn and normal pancreatic tissues were removed after 48 h to extract tissue fluid. Two-dimensional gel electrophoresis (2-DE) separation and spot analysis were conducted on differentially expressed proteins, and peptides were obtained after enzymatic digestion. Twenty two-fold or above differentially expressed proteins were identified via matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF/MS), among which, five proteins were related to pancreatic development and differentiation. Moreover, the expression patterns of four proteins detected w.. View More»
DOI:
10.4172/jpb.1000275