Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Gabriele Cevenini
Italy
Research Article
High Doses of Ascorbate Kill Y79 Retinoblastoma Cells In vitro
Author(s): Domenico Mastrangelo, Lauretta Massai, Lavinia Micheli, Michela Muscettola, Gabriele Cevenini and Giovanni GrassoDomenico Mastrangelo, Lauretta Massai, Lavinia Micheli, Michela Muscettola, Gabriele Cevenini and Giovanni Grasso
Objectives: To tests the sensitivity of Y79 retinoblastoma cell lines to high doses of ascorbate, in vitro, and compare its effects with those of some chemotherapeutic agents routinely employed in the treatment of retinoblastoma.Methods: Y79 retinoblastoma cells have been exposed to increasing doses of either sodium ascorbate (SA) or Melphalan (MEL), to define a dose-response curve around the peak plasma concentrations reached by both chemicals when administered according to the existing therapeutic procedures and protocols. The assessment of cell number and viability was performed, before and after exposure, with both the manual (Trypan Blue Exclusion Test) and automated (flow cytometry) methods. Fluorescence microscopy and direct observation of cells in culture, with inverted microscope, were also performed.Results:.. View More»
DOI:
10.4172/2155-9570.1000268