Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Gabriele Multhoff
Klinikum rechts der Isar, Technische Universität München,
Ismaninger Str. 22, 81675 Munich
Germany
Research Article
Quantitative Analysis of Liposomal Heat Shock Protein 70 (Hsp70) in the Blood of Tumor Patients Using a Novel LipHsp70 ELISA
Author(s): Stephanie Breuninger, Janina Erl, Clemens Knape, Sophie Gunther, Ivonne Regel, Franz Rödel, Udo S Gaipl, Jun Thorsteinsdottir, Lydia Giannitrapani, Anne M Dickinson and Gabriele MulthoffStephanie Breuninger, Janina Erl, Clemens Knape, Sophie Gunther, Ivonne Regel, Franz Rödel, Udo S Gaipl, Jun Thorsteinsdottir, Lydia Giannitrapani, Anne M Dickinson and Gabriele Multhoff
Elevated levels of the stress-inducible heat shock protein 70 (Hsp70) in the peripheral circulation have been reported for many tumor entities. In line with these findings we have shown that Hsp70 membrane-positive tumor cells actively release Hsp70 in exosome-like lipid vesicles. Since most commercial Hsp70 Enzyme-Linked Immunosorbent Assays (ELISAs) are not validated for the detection of liposomal Hsp70 in serum, the lipHsp70 ELISA was established using the monoclonal antibody cmHsp70.1 as a detection reagent. This antibody has been reported to recognize free and membrane-bound Hsp70 on living tumor cells. Validation of the ELISA showed a high assay precision and linearity in a concentration range of 0.36-17.4 ng/ml. A comparison of the recovery of spiked Hsp70 in buffer and serum samples revealed a significantly better recovery using the lipHsp70 .. View More»
DOI:
10.4172/2155-9899.1000264