Advanced Techniques in Biology & Medicine
Open Access
ISSN: 2379-1764
ISSN: 2379-1764
Galina Yu Lomakina
Serbia
Mini Review
Bioluminescence Methods for the Rapid Assay of the Specific Activity of Lyophilized BCG Vaccine
Author(s): Natalia N Ugarova and Galina Yu LomakinaNatalia N Ugarova and Galina Yu Lomakina
The specific activity of a lyophilized BCG vaccine (CFU/mg biomass) is one of the most important characteristics of this product. The classical microbiological method of the BCG vaccine activity assay is very time-consuming and gives results only in 4-5 weeks. The bioluminescent determination of specific activity based on the use of the firefly luciferase-luciferin system makes it possible to greatly shorten the duration of the assay. Since the 1970’s, the ATP method has been successively applied for studying the specific activity of different BCG strains. In 2008, the bioluminescent ATP method was proposed and evaluated for analyzing lyophilized BCG vaccine preparations. The duration of analysis decreased from several weeks to 2 days. This method was used in the International collaborative study to evaluate and establish WHO Reference Reagents for BCG vaccine. In 2016, a new pr.. View More»
DOI:
10.4172/2379-1764.1000243