Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Gennady Pogorelko
NI Vavilov Institute of General Genetics, Russian Academy of Sciences,
Gubkin Str., 3, 119991, Moscow
Serbia
Research Article
Identification and Analysis of the Arabidopsis Thaliana Atfas4 Gene Whose Overexpression Results in the Development of A Fasciated Stem
Author(s): Gennady Pogorelko, Oksana Fursova and Eugene Klimov
Gennady Pogorelko, Oksana Fursova and Eugene Klimov
Using a new pEnLox vector employed to generate gain-of-function mutants in Arabidopsis thaliana, the AtFAS4 mutant has been obtained and analyzed. The mutant is characterized by super-expression of the At1g33390 gene, which leads to the occurrence of a mutant phenotype – stem fasciation. The level of expression of the AtFAS4 gene in normally developing A.thaliana plants is extremely low thus accounting for almost complete absence of information on EST’s of this gene. The generated AtFAS4 mutant has permitted full-length cDNA of the At1g33390 gene to be obtained and analyzed for the first time.
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DOI:
10.4172/jpb.1000041