Journal of Proteomics & Bioinformatics
Open Access

Gianluca Bleve

Publications

• Research Article
  Study of a New Gliadin Capture Agent and Development of a Protein Microarray as a New Approach for Gliadin Detection
  Author(s): Fabio Cimaglia, Giovanni Potente, Maurizio Chiesa, Giovanni Mita and Gianluca BleveFabio Cimaglia, Giovanni Potente, Maurizio Chiesa, Giovanni Mita and Gianluca Bleve
  
  Celiac disease is an immune-mediated enteropathy precipitated by the ingestion of gluten-containing foods in genetically predisposed children and adults. After a positive diagnosis for celiac disease, the only available treatment is to adopt a gluten-free diet, and evaluation of the absence of gluten in foods is crucial for the health of celiac patients. In the present study, a recombinant glutamine-binding protein (GlnBP) from Escherichia coli showed its ability to recognize peptides deriving from digested wheat flour. GlnBP and the commercially available 4F3 monoclonal antibody, raised against a region of the α-gliadin peptide 33-mer from wheat, demonstrated the ability to detect gliadin extracted from wheat flour. Recombinant GlnBP and 4F3 monoclonal antibody were used as new capture agents for the development of a protein chip able to detect gluten in foods. The protein micr.. View More»
  DOI: 10.4172/jpb.1000326

  Abstract PDF

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