Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
GK Joshi
India
Research Article
Molecular Cloning of MAP Kinase Genes and In silico Identification of their Downstream Transcription Factors Involved in Pathogenesis of Karnal bunt(Tilletia indica) of Wheat
Author(s): Atul K Gupta, Anshita Goel, JM Seneviratne, GK Joshi and Anil KumarAtul K Gupta, Anshita Goel, JM Seneviratne, GK Joshi and Anil Kumar
The mitogen-activated protein kinase (MAPK) pathways control diverse cellular functions in pathogenic fungi, including sexual differentiation, stress response, and maintenance of cell wall integrity. Here we identified and characterized T indica MAPK genes, which are homologues to the other fungi viz. Pmk1 (Pathogenicity MAP Kinase-1) and Fus3 that is known to play an important role in pathogenicity and mating pheromone response during fungal development. It was performed through gene specific genomic DNA amplification, cloning, sequencing, homology match, annotation, motif analysis, structure prediction and protein-protein interaction. Comparative genomics approaches were applied for studying the role of such genes involved in pathogenesis. These analyses give new insights into similarities and dissimilarities as well as specific characteristics of T indica genom.. View More»
DOI:
10.4172/jpb.1000185