Journal of Proteomics & Bioinformatics
Open Access

Gopal Pande

Publications

• Research Article
  Proteomic Analysis Of The "Side Population" (SP) Cells From Murine Bone Marrow
  Author(s): Ravipati Satyavani, Azra Fatima, Curam S. Sundaram, Charumathi Anabalagan, CV Saritha, G Srinivas, Aleem Ahmed Khan, CM Habibullah and Gopal Pande Ravipati Satyavani, Azra Fatima, Curam S. Sundaram, Charumathi Anabalagan, CV Saritha, G Srinivas, Aleem Ahmed Khan, CM Habibullah and Gopal Pande
  
  Side population (SP) cells are a specialized cell type present at very low numbers in murine bone marrow and other tissues. These cells are considered to be multipotent stem cells capable of differentiating into several cell lineages in vivo and ex vivo. In this report we have isolated SP cells from suspensions of bone marrow of Balb-c mice by using a fluorescence-activated cell sorter (FACS) and we have done the comparative analysis of proteins expressed by the total bone marrow (TBM) and SP cells, by using standard procedures of 2D gel electrophoresis (2DE) followed by MALDI-MS and/or MS/MS. Immunofluorescence analysis of the expression of cytoskeletal proteins actin and vimentin and a multi drug resistance (MDR) family protein ABCG2 were done to further validate the biochemical proteomic data. The classification of the identified proteins into their functional cate.. View More»
  DOI: 10.4172/jpb.1000100

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