Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Gui-Wei He
China
Research Article
Signaling Networks in Gastric Cancer Cells Revealed by Phosphoproteomics
Author(s): Guang-Rong Yan, Nan-Peng Chen, Ya-Dong Huang, Wen Ding, Gui-Wei He, Chuan-Le Xiao, Xing-Feng Yin and Qing-Yu He
Guang-Rong Yan, Nan-Peng Chen, Ya-Dong Huang, Wen Ding, Gui-Wei He, Chuan-Le Xiao, Xing-Feng Yin and Qing-Yu He
Signaling molecules in signaling pathways proceed to be phosphorylated in the signal transmission from membrane receptors to nuclus. Dysregulated phosphorylation has been implicated in a variety of human diseases including cancer. In this study, we combined SDS-PAGE prefractionation, TiO2 phosphopeptide enrichment and LC-MS/MS technologies to identify the phosphoproteins and their regulatory sites in human gastric cancer cells. Totally 282 phosphorylation sites, corresponding to 245 unique peptides and 161 different proteins, were identified after the evaluation of ambiguous phosphosites. Among them, the phosphorylation of 109 (38.7%) sites and 36 (22.4%) proteins has not been previously reported. It was found that EGFR-ERK1/2 signaling network was mainly involved in the phosphorylation regulation of the identified proteins, suggestting that EGFR-ERK1.. View More»
DOI:
10.4172/jpb.1000129