Journal of Clinical and Cellular Immunology
Open Access

Halasa M

Publications

• Research Article
  Bone Marrow Lin-/Sca-1+/C-Kit+ and Lin-/Sca-1-/C-Kit+ cells Induce Stable Mixed Chimerism and Permanent Skin Graft Acceptance in a Mouse Model
  Author(s): Baśkiewicz-Hałasa M, Hałasa M, Dziedziejko V, Pius E and Machaliński BBaśkiewicz-Hałasa M, Hałasa M, Dziedziejko V, Pius E and Machaliński B
  
  Mixed chimerism is a potential induction method for alloantigen-specific tolerance. Although the phenomenon has been broadly studied over recent years, some important aspects remain to be elucidated. The aim of our study was to identify the optimal mouse stem/progenitor cell population for mixed chimerism induction purposes. To induce stable mixed chimerism, B6.SJL-PtprcaPep3b (CD45.1; H-2s; I-E-) mice were exposed to 3-Gy total body irradiation (Day -1) as well as the CD40L (Day 0, and 4) and CD8 (Day -2) blocking antibodies. The animals were transplanted with 10×106 Balb/c (CD45.2; H-2d; I-E+) unfractionated bone marrow cells (Day 0). Since mouse stem/progenitor cells are found among lin- population and they possess Sca-1 and c-kit antigens, mice were given 2×105 of lin-/Sca-1+/c-kit-, lin-/Sca-1+/c-kit+ or lin-/Sca-1-/c-kit+ bone marrow-derived cells. Mixed chimer.. View More»
  DOI: 10.4172/2155-9899.S9-004

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