Journal of Clinical and Cellular Immunology
Open Access

Hans Gaines

Publications

• Commentary
  Mycobacterium tuberculosis Vaccination Imprints on T-Cell Dynamics Associated with Mycobacterium tuberculosis Challenge in Rhesus Monkeys
  Author(s): Isabelle Magalhaes, Raija K. Ahmed, Nalini Vudattu, Donata R. Sizemore, Frank Weichold, Giulia Schirru, Maria Grazia Pau, Jaap Goudsmit, Alan Thomas, Frank Verreck, Ivanela Kondova, Jan Andersson, Yasir A.W. Skeiky, Jerry Sadoff, Hans Gaines, Rigmor Thorstensson, Mats Spångberg and Markus MaeurerIsabelle Magalhaes, Raija K. Ahmed, Nalini Vudattu, Donata R. Sizemore, Frank Weichold, Giulia Schirru, Maria Grazia Pau, Jaap Goudsmit, Alan Thomas, Frank Verreck, Ivanela Kondova, Jan Andersson, Yasir A.W. Skeiky, Jerry Sadoff, Hans Gaines, Rigmor Thorstensson, Mats Spångberg and Markus Maeurer
  
  Non-human primate models aid to design novel vaccine strategies. We analyzed therefore the dynamics of cellular immune responses induced by a bacille Calmette-Guérin (BCG) / recombinant BCG (rBCG, perfringolysin plus Ag85A/B, TB10.4) prime, subsequent adenoviral (Ad35 expressing Ag85A/B and TB10.4) boosts followed by a challenge with M. tuberculosis (Mtb, Erdman strain) in peripheral blood mononuclear cells from rhesus macaques. T-cell compartment mobilization was defined by CD45RA/CCR7/CD27/CD28 expression on CD4+, CD8αα+ and CD8αβ+ T-cells and functional analysis (STAT-5 phosphorylation) was carried out using multicolor flow cytometry. CD4+ as well as CD8+ T-cells showed a marked decrease in IL-7 receptor alpha-chain (IL-7Rα) expression in vaccinated animals (n=12/12) after the first adenoviral boost, but not in control animals (who received saline). BC.. View More»
  DOI: 10.4172/2155-9899.S4-006

  Abstract PDF

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