Journal of Applied Pharmacy
Open Access
ISSN: 1920-4159
+44 1300 500008
ISSN: 1920-4159
+44 1300 500008
Hasan MH
Egypt
Research Article
Design and Validation of Stability Indicating Assay of Glibenclamide Using
RP-HPLC Technique in Both Bulk, Pharmaceutical Formulations and Human Plasma
Author(s): El-Adl SM, El-Sadek ME and Hasan MH
El-Adl SM, El-Sadek ME and Hasan MH
A simple and sensitive high performance liquid chromatography method was developed for determination of glibenclamide in presence of its impurities in pharmaceutical dosage form and human plasma. Instrumentation of the method was very simple and used the mixture of acetonitrile: water (60:40, v/v) as the mobile phase. Separation was carried out on a BDS. Hypersil C8 (5 um, 250 × 4.6 mm) column. Effects of composition of mobile phase in addition to flow rate and detection wave length were studied. Calibration was obeyed in the range of 20-100 μg/ml of glibenclamide. The method was validated according to ICH parameters.
.. View More»
DOI:
10.21065/1920-4159.1000250