Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Hauge AW
Denmark
Commentary
Case Report: Anti-HLA-DQα Antibodies Have Clinical Impacts in Highly Immunized Kidney Patients
Author(s): Lund KP, Hauge AW, Sørensen SS and Bruunsgaard H
Lund KP, Hauge AW, Sørensen SS and Bruunsgaard H
Context and purpose: Organ exchange programmes are important in order to ensure organs to highly immunized kidney patients. In this case report, we describe a highly immunized patient in an acceptable mismatch program who had an unexpected positive Complement-Dependent Cytotoxicity (CDC) crossmatch with a deceased donor exchange.
Main findings: Subsequent evaluation revealed donor specific antibodies characterized by serological epitopes, which included the HLA-DQα chain, were masked in the standard IgG Luminex assay unless the serum was diluted; however, the antibodies demonstrated high MFI values when the serum was pretreated with EDTA or analyzed with the C1q assay. These findings led us to evaluate the extent of masked anti-HLA-DQ antibodies that included HLADQα/ DQβ pairs rather than just the HLA-DQβ chains in 16 high.. View More»
DOI:
10.4172/2155-9899.1000498