Journal of Drug Metabolism & Toxicology
Open Access

Hiroshi Sonoda

Publications

• Research Article
  Higher Expressions of Cytochrome P450, UDP-Glucuronosyltransferase, and Transporter Genes in Nanopillar-Cultured Rat Hepatocyte Spheroids
  Author(s): Ryosuke Takahashi, Akiko Hisada and Hiroshi Sonoda Ryosuke Takahashi, Akiko Hisada and Hiroshi Sonoda
  
  Primary hepatocytes have been widely explored as cell sources for the study of in vitro drug metabolism and pharmacokinetics (DMPK). Aiming toward establishing an in vitro drug screening method, the objective of the current study is to illustrate a comprehensive increase in the DMPK-related gene expression of nanopillar (NP)-cultured 3D-spheroids. To examine the expressional changes in DMPK-related genes under four different conditions, namely, NP-, sandwich (SW)-, monolayer (ML)-cultured rat hepatocytes, and freshly isolated hepatocytes, genome-wide gene-expression analysis using a DNA microarray was performed. Among the DMPK-related genes, cytochrome P450, UDP-glucuronosyltransferase, and transporter genes were focused on. Principal component analysis showed that the global gene expression profile in a sample from an NP culture was closer to that from freshly isolated hepatocytes than .. View More»
  DOI: 10.4172/2157-7609.1000146

  Abstract PDF

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