Biochemistry & Pharmacology: Open Access
Open Access

Hitoshi Mineo

Publications

• Research Article
  Relationship between Partition Coefficients of Carboxylic Acids and Changes in Osmotic Resistance in Rat Erythrocytes In vitro
  Author(s): Hitoshi Mineo Hitoshi Mineo
  
  We examined monocarboxylic and dicarboxylic acids and their partition coefficients for osmotic fragility (OF) in isolated red blood cells (RBCs) in rats. The dense packed RBC was incubated in a phosphate–NaCl buffer solution containing each carboxylic acid at 0 to 100 mM at 37ºC for 1 h. The RBC suspensions were transferred into the OF test tubes containing NaCl from 0.2 to 0.9%. The hemoglobin concentration was determined and NaCl concentration inducing 50% hemolysis was calculated as OF value. The OF in RBCs was dose-dependently increased by exposure to some of monocarboxylic acids possessing certain length of hydrocarbons with more than 4 carbons. A positive and statistically significant correlation was obtained between the partition coefficients and the degree of change in OF for monocarboxylic acids. Dicarboxylic acids corresponded with the monocarboxylic acids had eit.. View More»
  DOI: 10.4172/2167-0501.1000236

  Abstract PDF

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