Biochemistry & Pharmacology: Open Access
Open Access

Hitoshi Mineo

Publications

• Review Article
  Effects of Carboxylic Acids on Osmotic Resistance in Rat and Guinea Pig Red Blood Cells in vitro: The Relationship between Partition Coefficient and Changes in Osmotic Fragility
  Author(s): Hitoshi Mineo Hitoshi Mineo
  
  The osmotic fragility (OF) of isolated Red Blood cells (RBCs) in guinea pigs and rats was examined by monocarboxylic, dicarboxylic and their partition coefficients. A phosphate–NaCl buffer solution that comprises each carboxylic acid at the concentration ranging from 0 to 100 mM was used to incubate dense-packed RBCs at 37°C for 60 minutes. The RBC suspensions were moved into OF test tubes containing NaCl from 0.1 to 0.8%. The hemoglobin concentration was determined and was observed that NaCl concentration induced 50% hemolysis as the OF value. The OF was was increased by dose dependently the exposure to some monocarboxylic acids that possessed hydrocarbon chains of more than 4 carbons in length in case of rats but no increase was observed in guinea pig RBCs. A positive correlation was obtained between the partition coefficients and the degree of change in OF for monocarboxy.. View More»
  DOI: 10.4172/2167-0501.1000245

  Abstract PDF

Relevant Topics