Biochemistry & Pharmacology: Open Access
Open Access

Hitoshi Mineo

Publications

• Research Article
  Benzoic Acid and its Derivatives Increase Membrane Resistance to Osmotic Pressure in Isolated Sheep Erythrocytes
  Author(s): Hitoshi Mineo, Chinami Matsuda, Yukiko Suzuki, Saeri Takahashi, Akina Chiba and Rikako Kasai Hitoshi Mineo, Chinami Matsuda, Yukiko Suzuki, Saeri Takahashi, Akina Chiba and Rikako Kasai
  
  There have been many reports that osmotic fragility (OF) in red blood cells (RBCs) is a valuable tool for assessing the actions of various chemicals on the cell membrane in vitro. We determined the effects of benzoic acid and its derivatives on OF in sheep RBCs in vitro. Isolated sheep RBCs were exposed to these substances at 0-100 mM in a buffer solution for 1 h, and the 50% hemolysis was then determined by soaking in 0.1-0.8% NaCl solution. OF was determined as the NaCl concentration inducing 50% hemolysis which was colorimetrically measured by the released hemoglobin concentration. Benzoic acid decreased OF in a dose-dependent manner. Heptanoic acid and cyclohexane carboxylic acid, both of which have 6 carbons in their saturated hydrocarbon structure, did not change OF. Replacement of the COOH bound to the benzene ring with PO(OH)2 or SO2OH abolished the OF respon.. View More»
  DOI: 10.4172/2167-0501.1000260

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