Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-77-2385-9429
ISSN: 2167-0501
+44-77-2385-9429
Hmila I
Tunisia
Research Article
Effectiveness of the Androctonus Australis Hector Nanobody NbF12-10 Antivenom to Neutralize Significantly the Toxic Effect and Tissue Damage Provoked by Fraction of Androctonus mauretanicus (Morocco) Scorpion Venom
Author(s): Chgoury F, Benabderrazek R, Tounsi H, Oukkache N, Hmila I, Boubaker S, Ayeb ME, Saïle R, Ghalim N and Bouhaouala-Zahar BChgoury F, Benabderrazek R, Tounsi H, Oukkache N, Hmila I, Boubaker S, Ayeb ME, Saïle R, Ghalim N and Bouhaouala-Zahar B
Scorpion stings are life threatening in large parts of the world. Toxins from scorpion venom are responsible for severe metabolic and tissue disruption and immunotherapy is the only specific treatment able to neutralize the toxic effects of scorpion venom. The Androctonus mauretanicus (Am) is the most dangerous scorpion in Morocco, whereas in Tunisia Androctonus australis hector (Aah) is causing most casualties. In this work, we investigated the potential of NbF12-10, a new immunotherapeutic concept based on anti-toxin Nanobodies (Nbs) to neutralize Am scorpion venom. We first explored the immune cross-reactivity between Am and Aah scorpions venoms using anti-AahI and anti-AahII polyclonal, NbAahI’F12 (anti-AahI’), monospecific NbAahII10 (anti-AahII) and bispecific NbF12-10 (anti-AahI’/anti- AahII) monoclonal antibodies and subsequently we study the histological dama.. View More»
DOI:
10.4172/2167-0501.1000174