Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
+44 1300 500008
ISSN: 2155-9880
+44 1300 500008
Hong Chen
Shanghai Jiaotong University School of Medicine,
280 South Chong Qing Road, Shanghai 200025
China
Research Article
Effects of Hyperosmotic Sodium Chloride Perfusion on Ischemia/Reperfusion Injury in Isolated Hearts of Normal and Stroke-Prone Spontaneously Hypertensive Rats
Author(s): Wei-Li Shen, Li-Bing Chen, Jian-Xing Zhao, Shu-Jie Guo, Yi-Chen Chen, Li-Ping Wang, Yong Cao, Wen-Jun Yuan and Hong ChenWei-Li Shen, Li-Bing Chen, Jian-Xing Zhao, Shu-Jie Guo, Yi-Chen Chen, Li-Ping Wang, Yong Cao, Wen-Jun Yuan and Hong Chen
Background: Hyperosmotic solutions have been used successfully in different shock resuscitations with cardioprotection. This study was to examine the effects of hyperosmotic sodium chloride on isolated heart function and heart responses to ischemia/reperfusion in normotensive and hypertensive rats. The roles of hyperosmolarity-induced antioxidants including hyperosmolarity-relevant heat shock proteins as well as vasodilating endothelial nitric oxide synthase (eNOS) and vasoactive catecholamines were investigated.
Methods: Hearts of normal rats and stroke-prone spontaneously hypertensive rats were isolated and perfused for 30 min with control Krebs-Henseleit buffer (osmolarity 300 mOsm/L) or hyperosmotic buffer of different sodium chloride concentrations (320, 350 and 400 mOsm/L) before subjected to 40-min global ischemia followed by 10-min hype.. View More»
DOI:
10.4172/2155-9880.1000146