Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Hong Wei Chu
National Jewish Health,
1400 Jackson St., Room A639, Denver, CO 80206
Tanzania
Research Article
MUC18 Differentially Regulates Pro-Inflammatory and Anti-Viral Responses in Human Airway Epithelial Cells
Author(s): Reena Berman, Chunjian Huang, Di Jiang, James H. Finigan, Qun Wu and Hong Wei ChuReena Berman, Chunjian Huang, Di Jiang, James H. Finigan, Qun Wu and Hong Wei Chu
Objective: MUC18 or CD146, a transmembrane glycoprotein, is mainly expressed by endothelial cells and smooth muscle cells where it serves as a cell-cell adhesion molecule. We have found MUC18 up-regulation in airway epithelial cells of patients with asthma and chronic obstructive pulmonary disease (COPD). However, the function of MUC18 in airway epithelial cells remains unclear. In the present study, we tested the hypothesis that MUC18 exerts a pro-inflammatory function during stimulation with a viral mimic polyI:C or human rhinovirus infection.Methods: Normal human primary airway epithelial cells were transduced with lentivirus encoding MUC18 cDNA to over-express MUC18 or with GFP (control), and treated with polyI:C or HRV for detection of pro-inflammatory cytokine IL-8 and anti-viral gene IFN-β. Additionally, we performed cell culture of .. View More»
DOI:
10.4172/2155-9899.1000257