ISSN: 0974-276X
Huoming Zhang
Core Labs,
Thuwal, 23955-6900
Saudi Arabia
Research Article
Redox Proteome Perturbation in Arabidopsis upon Pseudomonas syringae Infection
Author(s): Pei Liu, Huoming Zhang, Liming Xiong and Yiji Xia
Pei Liu, Huoming Zhang, Liming Xiong and Yiji Xia
Oxidative burst is one of the earliest plant cellular responses triggered by pathogen infection. Reactive oxygen species can cause oxidative modifications of redox-sensitive proteins to mediate the defense responses. Identification and characterization of proteins that undergo oxidative modifications in these processes is an important step toward understanding molecular mechanisms of plant defense responses. In this study, an in vivo 15N metabolic labeling method combined with a cysteine-containing peptide enrichment technique was applied to identify and quantify proteins and their redox states in Arabidopsis in response to infection by Pseudomonas syringae pv tomato DC3000 (Pst). Changes of peptide redox states were compared and corrected with the changes of protein levels. A total of forty peptides representing thirty-six non-redundant proteins showed sign.. View More»
DOI:
10.4172/0974-276X.1000490