Advances in dairy Research
Open Access

HV Raghu

Publications

• Research Article
  Development of “Field Level” Chromogenic Assay for Aflatoxin M1 Detection in Milk
  Author(s): Neelam Verma, Namita Ashish Singh, Naresh Kumar, Vinai Kumar Singh and HV Raghu Neelam Verma, Namita Ashish Singh, Naresh Kumar, Vinai Kumar Singh and HV Raghu
  
  Out of eighteen Bacillus spp. screened for aflatoxin M1 (AFM1) sensitivity Bacillus brevis MTCC 3136 was found most sensitive followed by B. megaterium ATCC 9885 at 0.5 ppb (Codex limit). Bacillus brevis 3136 was found weakly positive for lipase activity while B. megaterium 9885 showed significant acetyl esterase activity. Maximum sporulation was achieved in Arret and Krishbaum sporulation agar (90.63%) followed by tryptone yeast extract broth, tryptone glucose yeast extract broth, minimal media and sucrose salt medium. A field level chromogenic assay for AFM1 in milk has been developed using spores of B. megaterium 9885 grown in Arret and Krishbaum sporulation agar. Spores were added with milk containing germinants (dextrose, sucrose, tryptone) and chromogenic substrate followed by incubation at 37˚C for 75 ± 5 min. The colour change from colourle.. View More»
  DOI: 10.4172/2329-888X.1000108

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