Journal of Horticulture
Open Access

Ichiro Kasajima

Publications

• Research Article
  Practical DNA markers to Estimate Apple (Malus x domestica Borkh.) Skin Color, Ethylene Production and Pathogen Resistance
  Author(s): Teppei Kikuchi, Ichiro Kasajima, Mizuki Morita and Nobuyuki Yoshikawa Teppei Kikuchi, Ichiro Kasajima, Mizuki Morita and Nobuyuki Yoshikawa
  
  Determination of agronomically important traits with DNA markers in genetically segregating populations is a key to high-throughput breeding of apple trees. In the present report, we first estimated the usefulness of four PCR-based DNA markers of apple in a set of 22 apple cultivars, which include popular cultivars in Japan such as ‘Fuji’, ‘Orin’, and ‘Shinano Gold’. The four DNA markers are reported to determine fruit storage life (ethylene production), fruit skin color, Alternaria resistance, and scab resistance. The target genes/locus of these DNA markers are MdACS1 gene, MdMYB1 gene, MdAlt locus, and MfVf gene. The types of these DNA markers are SSLP, dCAPS, allele-specific, and gene-specific, thus the markers were designated MdACS1-SSLP, MdMYB1-dCAPS(BstEII), MdAlt-AS, and MfVf-GS, respectively. DNA band patterns after gel electrophoresis were .. View More»
  DOI: 10.4172/2376-0354.1000211

  Abstract PDF

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